Xingwang Liu,1 Ang Tian,2 Junhua You,3 Hangzhou Zhang,4 Lin Wu,5 Xizhuang Bai,1 Zeming Lei,1 Xiaoguo Shi,2 Xiangxin Xue,2 Hanning Wang4 1Department of Orthopedics, The People’s Hospital of China Medical University, 2Liaoning Provincial Universities Key Laboratory of Boron Resource Ecological Utilization Technology and Boron Materials, Northea
An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes.
Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice.However, current protocols for electroporation either require the investment in specialized Headwear electroporators or corrosive pre-treatment of zygotes which compromises embryo viability.Here,
Estimation of zooplankton mortality caused by an Arctic glacier outflow
The outflow of freshwater from underwater Decoupage Paper channels in the Kongsbreen tidal glacier in Kongsfjorden, Svalbard, 79oN, was measured as 138.8 m3 s-1 at the peak of the melting season.Experiments on local marine plankton mortality show that when exposed to salinities below 9 PSU, all copepods die within 15 minutes.We estimate that during
Dental Implants in Patients with Oral Lichen Planus: A Systematic Review
Background and Objectives: To integrate the available published data on patients with oral lichen planus (OLP) rehabilitated with dental implants, as well as to review the recommendations for OLP patients receiving implants.Materials and Methods: An electronic search was undertaken in February 2019 using five databases.Publications reporting cases